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diffraction limited imaging  (Nikon)


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    Nikon diffraction limited imaging
    Diffraction Limited Imaging, supplied by Nikon, used in various techniques. Bioz Stars score: 99/100, based on 10098 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/diffraction+limited+imaging/pmc12892211-308-0-6?v=Nikon
    Average 99 stars, based on 10098 article reviews
    diffraction limited imaging - by Bioz Stars, 2026-06
    99/100 stars

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    Image Search Results


    Schematic diagram of the widefield epifluorescence Mesolens system.

    Journal: Journal of Biomedical Optics

    Article Title: Obtaining super-resolved images at the mesoscale through super-resolution radial fluctuations

    doi: 10.1117/1.JBO.29.12.126502

    Figure Lengend Snippet: Schematic diagram of the widefield epifluorescence Mesolens system.

    Article Snippet: In this work, we have demonstrated using SRRF in conjunction with diffraction-limited widefield Mesolens images, that it is possible to obtain super-resolved images at the mesoscale—achieving a resolution of 446.3 nm across a 4.4 × 3.0 mm FOV.

    Techniques:

    Complete Mesolens FOV showing the SRRF-processed image of the tubulin in HeLa cells labeled with AF488 with three highlighted ROI: magenta, cyan, and yellow (a). A digital zoom of the raw widefield epifluorescence diffraction limited Mesolens image is shown for each ROI (b)–(d), with a digital zoom of the improved ROI following SRRF processing (e)–(g). Scale bars are 30 μ m for all ROIs.

    Journal: Journal of Biomedical Optics

    Article Title: Obtaining super-resolved images at the mesoscale through super-resolution radial fluctuations

    doi: 10.1117/1.JBO.29.12.126502

    Figure Lengend Snippet: Complete Mesolens FOV showing the SRRF-processed image of the tubulin in HeLa cells labeled with AF488 with three highlighted ROI: magenta, cyan, and yellow (a). A digital zoom of the raw widefield epifluorescence diffraction limited Mesolens image is shown for each ROI (b)–(d), with a digital zoom of the improved ROI following SRRF processing (e)–(g). Scale bars are 30 μ m for all ROIs.

    Article Snippet: In this work, we have demonstrated using SRRF in conjunction with diffraction-limited widefield Mesolens images, that it is possible to obtain super-resolved images at the mesoscale—achieving a resolution of 446.3 nm across a 4.4 × 3.0 mm FOV.

    Techniques: Labeling

    Complete reconstructed SQUIRREL error map showing the areas of agreement between the raw widefield epifluorescence diffraction limited Mesolens image and the SRRF reconstruction (a). In the middle row, a digital zoom of the error map is displayed for each ROI (b)–(d), and in the bottom row, a contrast-adjusted ROI highlighting the areas of error (e)–(g). Scale bars are 30 μ m for all ROIs.

    Journal: Journal of Biomedical Optics

    Article Title: Obtaining super-resolved images at the mesoscale through super-resolution radial fluctuations

    doi: 10.1117/1.JBO.29.12.126502

    Figure Lengend Snippet: Complete reconstructed SQUIRREL error map showing the areas of agreement between the raw widefield epifluorescence diffraction limited Mesolens image and the SRRF reconstruction (a). In the middle row, a digital zoom of the error map is displayed for each ROI (b)–(d), and in the bottom row, a contrast-adjusted ROI highlighting the areas of error (e)–(g). Scale bars are 30 μ m for all ROIs.

    Article Snippet: In this work, we have demonstrated using SRRF in conjunction with diffraction-limited widefield Mesolens images, that it is possible to obtain super-resolved images at the mesoscale—achieving a resolution of 446.3 nm across a 4.4 × 3.0 mm FOV.

    Techniques:

    Complete Mesolens FOV showing the SRRF processed image of the GLUT-4 in 3T3-L1 adipocytes expressing GFP, with three highlighted ROI: magenta, cyan, and yellow (a). A digital zoom of the raw widefield epifluorescence diffraction limited Mesolens image is shown for each ROI (b)–(d), with a digital zoom of the improved ROI following SRRF processing below (e)–(g). Scale bars are 30 μ m for all ROIs.

    Journal: Journal of Biomedical Optics

    Article Title: Obtaining super-resolved images at the mesoscale through super-resolution radial fluctuations

    doi: 10.1117/1.JBO.29.12.126502

    Figure Lengend Snippet: Complete Mesolens FOV showing the SRRF processed image of the GLUT-4 in 3T3-L1 adipocytes expressing GFP, with three highlighted ROI: magenta, cyan, and yellow (a). A digital zoom of the raw widefield epifluorescence diffraction limited Mesolens image is shown for each ROI (b)–(d), with a digital zoom of the improved ROI following SRRF processing below (e)–(g). Scale bars are 30 μ m for all ROIs.

    Article Snippet: In this work, we have demonstrated using SRRF in conjunction with diffraction-limited widefield Mesolens images, that it is possible to obtain super-resolved images at the mesoscale—achieving a resolution of 446.3 nm across a 4.4 × 3.0 mm FOV.

    Techniques: Expressing

    Reconstructed SQUIRREL error map showing the areas of agreement between the raw widefield epifluorescence diffraction limited Mesolens image and the SRRF reconstruction with highlighted ROI: magenta, cyan, and yellow (a). A digital zoom of the error map is displayed for each ROI (b)–(d), with a contrast-adjusted ROI highlighting the areas of error within each ROI below (e)–(g). Scale bars are 30 μ m for each ROI.

    Journal: Journal of Biomedical Optics

    Article Title: Obtaining super-resolved images at the mesoscale through super-resolution radial fluctuations

    doi: 10.1117/1.JBO.29.12.126502

    Figure Lengend Snippet: Reconstructed SQUIRREL error map showing the areas of agreement between the raw widefield epifluorescence diffraction limited Mesolens image and the SRRF reconstruction with highlighted ROI: magenta, cyan, and yellow (a). A digital zoom of the error map is displayed for each ROI (b)–(d), with a contrast-adjusted ROI highlighting the areas of error within each ROI below (e)–(g). Scale bars are 30 μ m for each ROI.

    Article Snippet: In this work, we have demonstrated using SRRF in conjunction with diffraction-limited widefield Mesolens images, that it is possible to obtain super-resolved images at the mesoscale—achieving a resolution of 446.3 nm across a 4.4 × 3.0 mm FOV.

    Techniques:

    Schematic diagram of the widefield epifluorescence Mesolens system.

    Journal: Journal of Biomedical Optics

    Article Title: Obtaining super-resolved images at the mesoscale through super-resolution radial fluctuations

    doi: 10.1117/1.JBO.29.12.126502

    Figure Lengend Snippet: Schematic diagram of the widefield epifluorescence Mesolens system.

    Article Snippet: Macros were used within Fiji to split both the reference image and super-resolution SRRF image into tiles of equal sizes, with the required diffraction-limited reference image taken as the first diffraction-limited widefield Mesolens image from each stack.

    Techniques:

    Complete Mesolens FOV showing the SRRF-processed image of the tubulin in HeLa cells labeled with AF488 with three highlighted ROI: magenta, cyan, and yellow (a). A digital zoom of the raw widefield epifluorescence diffraction limited Mesolens image is shown for each ROI (b)–(d), with a digital zoom of the improved ROI following SRRF processing (e)–(g). Scale bars are 30 μ m for all ROIs.

    Journal: Journal of Biomedical Optics

    Article Title: Obtaining super-resolved images at the mesoscale through super-resolution radial fluctuations

    doi: 10.1117/1.JBO.29.12.126502

    Figure Lengend Snippet: Complete Mesolens FOV showing the SRRF-processed image of the tubulin in HeLa cells labeled with AF488 with three highlighted ROI: magenta, cyan, and yellow (a). A digital zoom of the raw widefield epifluorescence diffraction limited Mesolens image is shown for each ROI (b)–(d), with a digital zoom of the improved ROI following SRRF processing (e)–(g). Scale bars are 30 μ m for all ROIs.

    Article Snippet: Macros were used within Fiji to split both the reference image and super-resolution SRRF image into tiles of equal sizes, with the required diffraction-limited reference image taken as the first diffraction-limited widefield Mesolens image from each stack.

    Techniques: Labeling

    Complete reconstructed SQUIRREL error map showing the areas of agreement between the raw widefield epifluorescence diffraction limited Mesolens image and the SRRF reconstruction (a). In the middle row, a digital zoom of the error map is displayed for each ROI (b)–(d), and in the bottom row, a contrast-adjusted ROI highlighting the areas of error (e)–(g). Scale bars are 30 μ m for all ROIs.

    Journal: Journal of Biomedical Optics

    Article Title: Obtaining super-resolved images at the mesoscale through super-resolution radial fluctuations

    doi: 10.1117/1.JBO.29.12.126502

    Figure Lengend Snippet: Complete reconstructed SQUIRREL error map showing the areas of agreement between the raw widefield epifluorescence diffraction limited Mesolens image and the SRRF reconstruction (a). In the middle row, a digital zoom of the error map is displayed for each ROI (b)–(d), and in the bottom row, a contrast-adjusted ROI highlighting the areas of error (e)–(g). Scale bars are 30 μ m for all ROIs.

    Article Snippet: Macros were used within Fiji to split both the reference image and super-resolution SRRF image into tiles of equal sizes, with the required diffraction-limited reference image taken as the first diffraction-limited widefield Mesolens image from each stack.

    Techniques:

    Complete Mesolens FOV showing the SRRF processed image of the GLUT-4 in 3T3-L1 adipocytes expressing GFP, with three highlighted ROI: magenta, cyan, and yellow (a). A digital zoom of the raw widefield epifluorescence diffraction limited Mesolens image is shown for each ROI (b)–(d), with a digital zoom of the improved ROI following SRRF processing below (e)–(g). Scale bars are 30 μ m for all ROIs.

    Journal: Journal of Biomedical Optics

    Article Title: Obtaining super-resolved images at the mesoscale through super-resolution radial fluctuations

    doi: 10.1117/1.JBO.29.12.126502

    Figure Lengend Snippet: Complete Mesolens FOV showing the SRRF processed image of the GLUT-4 in 3T3-L1 adipocytes expressing GFP, with three highlighted ROI: magenta, cyan, and yellow (a). A digital zoom of the raw widefield epifluorescence diffraction limited Mesolens image is shown for each ROI (b)–(d), with a digital zoom of the improved ROI following SRRF processing below (e)–(g). Scale bars are 30 μ m for all ROIs.

    Article Snippet: Macros were used within Fiji to split both the reference image and super-resolution SRRF image into tiles of equal sizes, with the required diffraction-limited reference image taken as the first diffraction-limited widefield Mesolens image from each stack.

    Techniques: Expressing

    Reconstructed SQUIRREL error map showing the areas of agreement between the raw widefield epifluorescence diffraction limited Mesolens image and the SRRF reconstruction with highlighted ROI: magenta, cyan, and yellow (a). A digital zoom of the error map is displayed for each ROI (b)–(d), with a contrast-adjusted ROI highlighting the areas of error within each ROI below (e)–(g). Scale bars are 30 μ m for each ROI.

    Journal: Journal of Biomedical Optics

    Article Title: Obtaining super-resolved images at the mesoscale through super-resolution radial fluctuations

    doi: 10.1117/1.JBO.29.12.126502

    Figure Lengend Snippet: Reconstructed SQUIRREL error map showing the areas of agreement between the raw widefield epifluorescence diffraction limited Mesolens image and the SRRF reconstruction with highlighted ROI: magenta, cyan, and yellow (a). A digital zoom of the error map is displayed for each ROI (b)–(d), with a contrast-adjusted ROI highlighting the areas of error within each ROI below (e)–(g). Scale bars are 30 μ m for each ROI.

    Article Snippet: Macros were used within Fiji to split both the reference image and super-resolution SRRF image into tiles of equal sizes, with the required diffraction-limited reference image taken as the first diffraction-limited widefield Mesolens image from each stack.

    Techniques:

    Schematic diagram of the widefield epifluorescence Mesolens system.

    Journal: Journal of Biomedical Optics

    Article Title: Obtaining super-resolved images at the mesoscale through super-resolution radial fluctuations

    doi: 10.1117/1.JBO.29.12.126502

    Figure Lengend Snippet: Schematic diagram of the widefield epifluorescence Mesolens system.

    Article Snippet: Here, we demonstrate that by applying SRRF, in conjunction with diffraction-limited widefield Mesolens imaging, as seen in , it is possible to obtain super-resolved images over a large multi-millimeter FOV through the analysis of intensity fluctuations across widefield Mesolens images.

    Techniques:

    Complete Mesolens FOV showing the SRRF-processed image of the tubulin in HeLa cells labeled with AF488 with three highlighted ROI: magenta, cyan, and yellow (a). A digital zoom of the raw widefield epifluorescence diffraction limited Mesolens image is shown for each ROI (b)–(d), with a digital zoom of the improved ROI following SRRF processing (e)–(g). Scale bars are 30 μ m for all ROIs.

    Journal: Journal of Biomedical Optics

    Article Title: Obtaining super-resolved images at the mesoscale through super-resolution radial fluctuations

    doi: 10.1117/1.JBO.29.12.126502

    Figure Lengend Snippet: Complete Mesolens FOV showing the SRRF-processed image of the tubulin in HeLa cells labeled with AF488 with three highlighted ROI: magenta, cyan, and yellow (a). A digital zoom of the raw widefield epifluorescence diffraction limited Mesolens image is shown for each ROI (b)–(d), with a digital zoom of the improved ROI following SRRF processing (e)–(g). Scale bars are 30 μ m for all ROIs.

    Article Snippet: Here, we demonstrate that by applying SRRF, in conjunction with diffraction-limited widefield Mesolens imaging, as seen in , it is possible to obtain super-resolved images over a large multi-millimeter FOV through the analysis of intensity fluctuations across widefield Mesolens images.

    Techniques: Labeling

    Complete reconstructed SQUIRREL error map showing the areas of agreement between the raw widefield epifluorescence diffraction limited Mesolens image and the SRRF reconstruction (a). In the middle row, a digital zoom of the error map is displayed for each ROI (b)–(d), and in the bottom row, a contrast-adjusted ROI highlighting the areas of error (e)–(g). Scale bars are 30 μ m for all ROIs.

    Journal: Journal of Biomedical Optics

    Article Title: Obtaining super-resolved images at the mesoscale through super-resolution radial fluctuations

    doi: 10.1117/1.JBO.29.12.126502

    Figure Lengend Snippet: Complete reconstructed SQUIRREL error map showing the areas of agreement between the raw widefield epifluorescence diffraction limited Mesolens image and the SRRF reconstruction (a). In the middle row, a digital zoom of the error map is displayed for each ROI (b)–(d), and in the bottom row, a contrast-adjusted ROI highlighting the areas of error (e)–(g). Scale bars are 30 μ m for all ROIs.

    Article Snippet: Here, we demonstrate that by applying SRRF, in conjunction with diffraction-limited widefield Mesolens imaging, as seen in , it is possible to obtain super-resolved images over a large multi-millimeter FOV through the analysis of intensity fluctuations across widefield Mesolens images.

    Techniques:

    Complete Mesolens FOV showing the SRRF processed image of the GLUT-4 in 3T3-L1 adipocytes expressing GFP, with three highlighted ROI: magenta, cyan, and yellow (a). A digital zoom of the raw widefield epifluorescence diffraction limited Mesolens image is shown for each ROI (b)–(d), with a digital zoom of the improved ROI following SRRF processing below (e)–(g). Scale bars are 30 μ m for all ROIs.

    Journal: Journal of Biomedical Optics

    Article Title: Obtaining super-resolved images at the mesoscale through super-resolution radial fluctuations

    doi: 10.1117/1.JBO.29.12.126502

    Figure Lengend Snippet: Complete Mesolens FOV showing the SRRF processed image of the GLUT-4 in 3T3-L1 adipocytes expressing GFP, with three highlighted ROI: magenta, cyan, and yellow (a). A digital zoom of the raw widefield epifluorescence diffraction limited Mesolens image is shown for each ROI (b)–(d), with a digital zoom of the improved ROI following SRRF processing below (e)–(g). Scale bars are 30 μ m for all ROIs.

    Article Snippet: Here, we demonstrate that by applying SRRF, in conjunction with diffraction-limited widefield Mesolens imaging, as seen in , it is possible to obtain super-resolved images over a large multi-millimeter FOV through the analysis of intensity fluctuations across widefield Mesolens images.

    Techniques: Expressing

    Reconstructed SQUIRREL error map showing the areas of agreement between the raw widefield epifluorescence diffraction limited Mesolens image and the SRRF reconstruction with highlighted ROI: magenta, cyan, and yellow (a). A digital zoom of the error map is displayed for each ROI (b)–(d), with a contrast-adjusted ROI highlighting the areas of error within each ROI below (e)–(g). Scale bars are 30 μ m for each ROI.

    Journal: Journal of Biomedical Optics

    Article Title: Obtaining super-resolved images at the mesoscale through super-resolution radial fluctuations

    doi: 10.1117/1.JBO.29.12.126502

    Figure Lengend Snippet: Reconstructed SQUIRREL error map showing the areas of agreement between the raw widefield epifluorescence diffraction limited Mesolens image and the SRRF reconstruction with highlighted ROI: magenta, cyan, and yellow (a). A digital zoom of the error map is displayed for each ROI (b)–(d), with a contrast-adjusted ROI highlighting the areas of error within each ROI below (e)–(g). Scale bars are 30 μ m for each ROI.

    Article Snippet: Here, we demonstrate that by applying SRRF, in conjunction with diffraction-limited widefield Mesolens imaging, as seen in , it is possible to obtain super-resolved images over a large multi-millimeter FOV through the analysis of intensity fluctuations across widefield Mesolens images.

    Techniques: